Search result for '27945 '.
Viewing records 3451 to 3500 of 3705 hits.
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N2105291
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Name: SALK_209427C |
Price:
£11.00
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Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105293
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Name: SALK_209431C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105294
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Name: SALK_209433C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105295
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Name: SALK_209434C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
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Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105297
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Name: SALK_209436C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
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Material type: seed
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105298
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Name: SALK_209438C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
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Material type: seed
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105299
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Name: SALK_209439C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
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Material type: seed
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105301
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Name: SALK_209441C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105302
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Name: SALK_209443C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105303
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Name: SALK_209444C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105304
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Name: SALK_209446C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105306
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Name: SALK_209448C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105307
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Name: SALK_209449C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105309
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Name: SALK_209451C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105310
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Name: SALK_209454C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105311
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Name: SALK_209455C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105312
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Name: SALK_209459C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105313
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Name: SALK_209460C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105314
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Name: SALK_209462C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105316
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Name: SALK_209464C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105317
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Name: SALK_209465C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105318
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Name: SALK_209466C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105319
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Name: SALK_209470C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105320
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Name: SALK_209472C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105321
|
Name: SALK_209473C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
Locus
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please acknowledge ABRC for distributing the seeds in all presentations and publications utilizing this material.
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N2105322
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Name: SALK_209474C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105323
|
Name: SALK_209475C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105324
|
Name: SALK_209476C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105325
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Name: SALK_209477C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105326
|
Name: SALK_209480C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105327
|
Name: SALK_209481C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105328
|
Name: SALK_209482C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105329
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Name: SALK_209484C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating.
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Phenotype
no phenotype information available at this time
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N2105330
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Name: SALK_209488C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105332
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Name: SALK_209493C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105333
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Name: SALK_209495C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105334
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Name: SALK_209498C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105335
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Name: SALK_209500C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105336
|
Name: SALK_209501C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105337
|
Name: SALK_209502C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105338
|
Name: SALK_209504C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105339
|
Name: SALK_209506C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105340
|
Name: SALK_209508C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105341
|
Name: SALK_209509C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105342
|
Name: SALK_209510C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105343
|
Name: SALK_209513C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
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Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105344
|
Name: SALK_209520C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105345
|
Name: SALK_209526C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105346
|
Name: SALK_209530C |
Price:
£11.00
|
Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
|
|
Stock type: individual line
|
Material type: seed
|
|
Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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N2105347
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Name: SALK_209534C |
Price:
£11.00
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Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Stock type: individual line
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Material type: seed
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Description T-DNA insertion line; confirmed line isolated from original SALK population; presence of the insertion was identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line.
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